Award details

sig1, a central regulator of environmental stress responses in Streptomyces coelicolor

Reference	BBS/E/J/00004401
Principal Investigator / Supervisor	Professor Mark Buttner
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	75,022
Status	Completed
Type	Institute Project
Start date	01/09/1999
End date	31/03/2004
Duration	55 months

Abstract

We have identified a locus (sig1) that functions as a central regulator of stress responses in S. coelicolor and, in collaboration with Prof. Charles Thompson, we have shown that two phi factors, phi37 and phi52, are produced from the sig1 gene, arising from alternative translation start codons. The aims of the proposed work are (i) to use proteome analysis to identify proteins whose expression is controlled by phi37 and/or phi52 (ii) to investigate the role of orfX in the regulation of phi37/52 activity (iii) to demonstrate Prs1 anti-sigma factor activity and to investigate its interaction with both phi52 and phi37 (iv) to identify the mechanism which regulates the activity of Prs1 (v) to understand the negative role of sig1 in the regulation of CatA expression, and (vi) to begin to investigate the roles of other identified B. subtilis phiB-like phi factors in S. coelicolor.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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