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Sensing and responding to cell envelope stress in streptomyces

Reference	BBS/E/J/00004205
Principal Investigator / Supervisor	Professor Mark Buttner
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	33,531
Status	Completed
Type	Institute Project
Start date	01/10/2001
End date	30/09/2004
Duration	36 months

Abstract

We have identified a signal transduction system that modulates expression of cell envelope-related genes in response to cell envelope stress in S. coelicolor. The system is comprised of four proteins; sigma-E, an RNA polymerase sigma factor; CseB, a response regulator; CseC, a sensor kinase; and CseA, a negative regulator of unknown biochemical function. The aims of the proposed work are to define (i) the biochemical function and (ii) the subcellular location of CseA (iii) to analyse the eps operon under E control (iv) to characterise the product specified by the eps operon (v) to define the CseB binding site(s) on the sigE promoter (vi) to identify further genes under sigma-E control, and (vii) to improve the commercial potential of the sigE system as the basis for a broad-range, generic screen for cell envelope-specific antibiotics.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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