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Molecular interactions between Marek's disease virus-encoded proteins and cell-cycle proteins in oncogenesis

Reference	BBS/E/I/00000898
Principal Investigator / Supervisor	Professor Venugopal Nair
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	6,270
Status	Completed
Type	Institute Project
Start date	01/10/2001
End date	30/09/2004
Duration	36 months

Abstract

Marekthats disease (MD), is a neoplastic disease of poultry caused by a highly contagious, cell-associated herpesvirus. The molecular mechanisms of MDV-induced oncogenesis are not completely understood. Many DNA tumour viruses interfere with the cell cycle regulation during oncogenesis. There is very little data on the association between the cell cycle regulation and the induction of T cell lymphomas in MD. However, recent observations on mutations in the p53 gene in MD tumours as well as the interaction of MDV-encoded protein meq with p53 and retinoblastoma protein (pRb), suggested that MDV also should be targeting the cell cycle during the tumourigenesis. This proposed project is to examine (i) whether MDV does influence the cell cycle (ii) what are the cell cycle regulatory targets of the virus, and (iii) which virus-encoded proteins interact with the cell cycle regulation. The project will make use of the molecular biological techniques such as cell cycle analysis and confocal microscopy, construction of recombinant viral and cell cycle regulatory proteins, protein-protein interactions including yeast two-hybrid systems, glutathione-S- transferase (GST) pull down assays. It is hoped that these studies will help to understand the mechanisms of MD oncogenesis and identify the viral and cellular gene(s) involved in oncogenic pathway.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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