Award details

Development of mouse models for the study of human TSEs

Reference	BBS/E/I/00000654
Principal Investigator / Supervisor	Professor Jean Manson
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	The Pirbright Institute Department
Funding type	Research
Value (£)	41,589
Status	Completed
Type	Institute Project
Start date	01/08/1997
End date	31/07/2000
Duration	36 months

Abstract

Transgenic mice in which the mouse PrP gene has been replaced with a human PrP gene will be produced to enable the development of murine models for the study of human spongiform encephalopathies and the experimental assessment of the susceptibility of man to various strains of CJD, BSE and scrapie. Gene targeting techniques will be used to replace the coding region of the endogenous murine PrP gene with that of the human PrP gene. This proposal envisages using two different 'wild-type' allelic forms of the human gene for the targeting experiments, one with Met at codon 129 (Met129) and the other with Valine (Val129). Targeting constructs carrying the human gene will be electroporated into HM1 Murine embryonic stem cells. Targeted clones will be identified by selection in thioguanine and confirmed by PCR and Southern analysis. Clones in which one copy of the murine gene has been replaced by the human gene will be injected into blastocysts and reimplanted into foster mothers for the production of chimeric mice. The chimeras will be bred to produce mice homozygous for the introduced human PrP gene. The Met129 and Val129 homozygous lines will be crossed to produce heterozygous Met129 and Val129 mice.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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