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Role of PrP in normal development & neurodegeneration in novel transgenic mice in which PrP gene expression is temporally & spatially controlled
Reference
BBS/E/I/00000647
Principal Investigator / Supervisor
Professor Jean Manson
Co-Investigators /
Co-Supervisors
Institution
The Pirbright Institute
Department
The Pirbright Institute Department
Funding type
Research
Value (£)
46,427
Status
Completed
Type
Institute Project
Start date
08/01/1998
End date
07/01/2003
Duration
60 months
Abstract
We propose to generate two new lines of PrP mutant mice in which normal PrP expression will be controlled temporally using the bacteriophage P1 cre/loxP site specific recombination system. Single rounds of gene targeting will be used, followed by in vitro selection, to produce two embryonic stem cell lines. One of these will carry two lox sites flanking PrP exonic sequences and one will carry a lox flanked stop cassette prior to exon 3. Subsequent intercrossing with animals transgenic for an inducible form of Cre recombinase will produce strains in which it will be possible to functionally activate or inactivate PrP by expressing Cre recombinase.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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