Award details

Analysis of Salmonella gene expression during the early stages of growth

Reference	BBS/E/F/00041765
Principal Investigator / Supervisor	Professor Jay Hinton
Co-Investigators / Co-Supervisors	Professor Michael William Peck
Institution	Quadram Institute Bioscience
Department	Quadram Institute Bioscience Department
Funding type	Research
Value (£)	1,063
Status	Completed
Type	Institute Project
Start date	01/10/2003
End date	30/09/2006
Duration	36 months

Abstract

Salmonella are important foodborne pathogens, being responsible for a serious and sometimes fatal disease. Predictive microbiology is a crucial tool for controlling growth of foodborne pathogens. A weakness in current predictive models is prediction of the early phase of growth (i.e. lag phase). At IFR, there is currently a large research programme led by Prof. Mike Peck and Dr. József Baranyi aimed at improved understanding and improved prediction of the lag phase of growth (see http://www.ifr.ac.uk/bacanova/ and http://www.ifr.ac.uk/safety/BehaviourPred.html).The purpose of this CASE studentship (with CCFRA) is to contribute to this research programme by extending understanding of the effect of salt on the lag phase of growth of Salmonella Typhimurium. This will be achieved by the use of microarray technology and gfp-reporter genes, and carried out under the supervision of Dr. Jay Hinton. This project will build on the expertise on Salmonella functional genomics at IFR (http://www.ifr.bbsrc.ac.uk/Safety/molmicro/research_microarrays.html). The student will study gene expression using DNA microarrays as bacterial cells are moved from stationary phase to lag phase under non-stressed and stressed conditions. The variability of lag time will also be assessed with the Bioscreen apparatus. Mathematical modelling approaches will be used to aid data analysis.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search