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Towards an integrated understanding of neutrophil biology and their role in resisting infection

Reference	BBS/E/B/000C0411
Principal Investigator / Supervisor	Professor Len Stephens
Co-Investigators / Co-Supervisors	Dr Phillip Hawkins, Dr Heidi Welch
Institution	Babraham Institute
Department	Babraham Institute Department
Funding type	Research
Value (£)	3,762,311
Status	Completed
Type	Institute Project
Start date	01/04/2012
End date	31/03/2017
Duration	59 months

Abstract

We will have started to exploit imaging tools (eg Raichu-Rac reporter mouse strain) and have progressed our systematic gene-targetting strategy to understand spatio-temporal regulation of PI3Ks and small GTPases that is known to be essential for neutrophil migration and pathogen-killing. This activity should lead to 2 or 3 papers being either accepted or under review at the mid term review. We will have established the cell lines expressing Fc Rs and GFP-iPX construct to enable us to commence a genome-wide screen for regulators of vps34 in the context of phagosome formation in comparison to our on-going screen for regulators of vps34 upstream of autophagosome formation (see below). We will have begun to define the primary transcriptional networks of mouse neutrophils and how that is determined by different stimuli in preparation for our studies to address how some aspects of the reduced functionality of neutrophils in aged animals maybe related to changes in their transcriptional landscape. We will validate the tools to probe the identity of endogenous protein cysteine residues modified by ROS in work with other cell types and apply them to neutrophils in the later stages of the ISPG

Summary

unavailable

Committee	Not funded via Committee
Research Topics	Immunology
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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