Award details

Ultrafast folding of PGK using laser photolysis

Reference	BBS/B/10218
Principal Investigator / Supervisor	Professor Jon Waltho
Co-Investigators / Co-Supervisors	Professor G Beddard, Professor Christopher Hunter, Professor Gareth Jones, Dr Gavin Reid
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	291,667
Status	Completed
Type	Research Grant
Start date	01/03/2004
End date	30/09/2007
Duration	43 months

Abstract

The aim is to exploit our newly developed methodology for photocaging proteins to examine protein folding on ultrafast timescales. We will use our cross-linking reagents to constrain proteins in non-native conformations and initiate folding reactions using laser flash photolysis. A unique feature of this approach is that we will be able to vary the starting conformation in a controlled manner, characterise its structure with very high positional resolution using heteronuclear NMR methods, and synchronise all molecules in this state. We will be in a position to answer very specific questions about the nature of the free energy landscape that controls protein folding reactions, down to picosecond timescales.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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