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Relaxation of electron spins by anisotropic paramagnetic metal centres; a novel probe of protein structure and dynamics

Reference	BBS/B/03637
Principal Investigator / Supervisor	Professor Andrew Thomson
Co-Investigators / Co-Supervisors	Dr Myles Cheesman
Institution	University of East Anglia
Department	Chemistry
Funding type	Research
Value (£)	227,361
Status	Completed
Type	Research Grant
Start date	01/08/2004
End date	30/09/2007
Duration	38 months

Abstract

We propose new methods, using time domain, dual frequency EPR, to determine distances and angles between a nitroxide spin label and a fast relaxing paramagnetic metal ion within a protein. New theoretical formalisms and simulation will allow analysis of motions of the label and protein domains. Validation will be carried out by measurement of longitudinal spin relaxation times, T1, at 9 and 94 GHz, from proteins of known structure labelled to provide a set of distances and angles between a spin label and a rapidly relaxing metal centre including heme, a [2Fe-2S]2+/1+ cluster, non-heme iron (III) and lanthanide(III) ions for ultra-fast relaxation. Lanthanide complexes covalently attached to a cysteine residue will allow determination of distance-angles between two differentially labelled proteins. Application to proteins in membranes and in whole cells will be possible.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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