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The role of the cajal body viral gene expression

Reference	BBS/B/03300
Principal Investigator / Supervisor	Professor George Blair
Co-Investigators / Co-Supervisors	Dr John Harold Walker
Institution	University of Leeds
Department	Inst of Molecular & Cellular Biology
Funding type	Research
Value (£)	205,565
Status	Completed
Type	Research Grant
Start date	01/07/2004
End date	30/06/2007
Duration	36 months

Abstract

We have discovered that in the early phase of adenovirus (Ad) infection of human cells, E1A proteins co-localise with Cajal bodies (CBs). We will map the domain of E1A that mediates the interaction with CBs using a panel of recombinant Ads containing small, in-frame deletions in E1A. The CB protein interacting with E1A will be defined by reversible protein cross-linking using CBs purified from Ad-infected cells followed by deletion mutagenesis. The importance of the E1A: Cajal interaction for establishment of productive viral infection will be investigated by targeted disruption techniques. We will analyse the dynamic association between E1A and the CB using time-lapse microscopy techniques. Finally we will also investigate whether transcriptional regulatory proteins of other DNA tumour viruses (namely HPV and SV40) interact with the Cajal body.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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