Award details

Next-generation bio-orthogonal reaction platform for RNA tagging and imaging

Reference	BB/T017104/1
Principal Investigator / Supervisor	Professor Glenn Burley
Co-Investigators / Co-Supervisors
Institution	University of Strathclyde
Department	Pure and Applied Chemistry
Funding type	Research
Value (£)	201,637
Status	Completed
Type	Research Grant
Start date	01/11/2020
End date	31/01/2022
Duration	15 months

Abstract

unavailable

Summary

The overall objective of this Follow-on-Fund (FoF) proposal is to establish the commercial potential of a new imaging platform to detect gene expression. For this proof of concept study, we will focus on the development of a new generation of RNA imaging agents, which we call 'click-aptazymes'. Underpinning this work is the patented discovery of small molecules which undergo a selective chemical reaction with each other under biologically-relevant conditions to form a fluorescent product. We now wish to demonstrate the potential to increase the rate of this reaction catalyzed by an RNA molecule when expressed in a cell. This RNA imaging approach would provide distinct sensitivity advantages of existing imaging technologies if proof of concept experiments show that it is functional in a cellular environment. In order to establish a route to market for this new technology, fundamental knowledge concerning the ability of RNA to catalyse the reaction, and the toxicity and cell uptake of the requisite components is essential. We envisage that the combined technical data and user engagement activities will streamline the commercial exploitation of these reagents in the pharmaceutical and biotechnology sectors.

Committee	Not funded via Committee
Research Topics	Technology and Methods Development
Research Priority	X – Research Priority information not available
Research Initiative	Follow-On Fund (FOF) [2004-2015]
Funding Scheme	X – not Funded via a specific Funding Scheme

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