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Quantative assessment of molecular recognition events by isothermal titration calorimetry

Reference	BB/C511313/1
Principal Investigator / Supervisor	Professor Florian Hollfelder
Co-Investigators / Co-Supervisors	Professor Sir Tom Blundell, Professor Paul Dupree, Professor Richard Farndale, Professor Nicholas Gay, Professor Christopher Howe, Professor Marko Hyvonen, Professor Ben Luisi, Dr Helen Mott, Dr Dareca Owen
Institution	University of Cambridge
Department	Biochemistry
Funding type	Research
Value (£)	42,438
Status	Completed
Type	Research Grant
Start date	13/12/2004
End date	12/12/2005
Duration	12 months

Abstract

Isothermal titration calorimetry (ITC) is a uniquely powerful tool for determining thermodynamic parameters of intermolecular interactions. A single experiment gives DG, DS and DH of binding and also the stoichiometry of interaction. ITC can also be used to follow the progress of reaction, thus providing a universal assay for any enzyme reaction. The requested equipment will be centrally managed and used primarily by the research groups listed as applicants, but will be made available to other users in the Cambridge community on an ad-hoc basis. The systems to be studied include: multiprotein complexes, enzyme inhibitors, ligand docking, mechanistic enzymology, novel antibiotic development and engineered proteins. There is great demand for this instrument which we do not have and urgently need.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Research Equipment Initiative 2004 (RE4) [2004]
Funding Scheme	X – not Funded via a specific Funding Scheme

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