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Probing molecular mechanisms of neurodegenerative ageing in prion ablated mice

ReferenceBB/C506356/2
Principal Investigator / Supervisor Dr Andrew Gill
Co-Investigators /
Co-Supervisors		 Dr Janet Fraser, Professor Jean Manson
Institution The Roslin Institute
DepartmentThe Roslin Institute Department
Funding typeResearch
Value (£) 173,567
StatusCompleted
TypeResearch Grant
Start date 01/04/2007
End date 30/04/2008
Duration13 months

Abstract

The transmissible spongiform encephalopathies (TSEs) are associated with accumulation of a protease-resistant and conformationally altered isoform, PrPSc, of the cellular prion protein, PrPC. These diseases are also associated with widespread spongiform degeneration, neuronal loss and astrocytic gliosis and are invariably fatal. It is not clear, however, whether neurodegeneration represents a response to increasing levels of neurtoxic PrPSc (so called gain of function) or is the result of loss of function of endogenous PrPC as it is converted to PrPSc. Mice in which the Prn-p gene has been ablated by gene targeting (PrP0/0) appear largely physiologically normal with no developmental abnormalities, suggesting that loss of function of PrPC may not account for changes seen during TSE disease. However, we have preliminary evidence for significant and dramatic changes in the abundance of certain proteins in the brains of PrP0/0 mice that have been aged to around 825 days, relative to their age-matched wildtype counterparts. These changes appear similar to those seen during TSE-induced neurodegeneration and our data suggest common molecular mechanisms in these two models, indicating that loss of PrPC function may be the pathophysiological event in TSE diseases. Our data suggest that PrPC plays a key role in protection of neurones during the ageing process. We wish to follow up our preliminary micro-array experiments and to extend this study to encompass proteomic and immunohistochemical approaches and propose a concerted programme of work aimed at defining the molecular mechanisms of cerebral dysfunction in PrP0/0 mice. Joint with BB/C506872/1

Summary

unavailable
Committee Closed Committee - Animal Sciences (AS)
Research TopicsAgeing, Animal Health, Neuroscience and Behaviour, TSEs (transmissible spongiform encephalopathies)
Research PriorityX – Research Priority information not available
Research Initiative Proteomics and Cell Function (PCF) [2003-2004]
Funding SchemeX – not Funded via a specific Funding Scheme
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