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Fumarate reductase: understanding the mechanism of proton and electron delivery

Reference	B19725
Principal Investigator / Supervisor	Professor Stephen Chapman
Co-Investigators / Co-Supervisors	Professor Graeme Reid, Professor Malcolm Walkinshaw
Institution	University of Edinburgh
Department	Sch of Chemistry
Funding type	Research
Value (£)	185,652
Status	Completed
Type	Research Grant
Start date	01/10/2003
End date	30/11/2006
Duration	38 months

Abstract

This proposal aims to completely elucidate (to atomic detail) the mechanisms for proton and electron delivery in the soluble fumarate reductase from Shewanella. Firstly, the inter-protein reaction between fumarate reductase and its physiological partner, the membrane bound cytochrome CymA, will be examined and explained. Secondly, we shall use kinetic and structural methods to determine the importance of the four heme groups in fumarate reductase for electron delivery in the flavin. Thirdly, we shall utilise solvent kinetic isotope effects and proton inventories to explain the importance of proton transfer in controlling the rate of fumarate reduction. We shall thus provide a unified picture of catalysis starting from electron delivery from the protein donor through to hydride transfer and proton transfer to fumarate at the active site of the enzyme.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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