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The aminocoumarin biosynthetic gene clusters of Streptomyces: structural and functional analysis

Reference	B19400
Principal Investigator / Supervisor	Professor David Lawson
Co-Investigators / Co-Supervisors	Dr Marcus Durrant, Professor Anthony Maxwell
Institution	John Innes Centre
Department	Biological Chemistry
Funding type	Research
Value (£)	214,676
Status	Completed
Type	Research Grant
Start date	01/12/2003
End date	30/11/2006
Duration	36 months

Abstract

The aminocoumarin antibiotics novobiocin, chlorobiocin and coumermycin A1 are secondary metabolites from Streptomyces that specifically inhibit the bacterial enzyme DNA gyrase. The gene clusters encoding their biosynthetic pathways have been cloned and sequenced. Plausible roles have been assigned to many of these genes, whilst others encode proteins of no known function. Concentrating principally on the novobiocin pathway, we will use a combination of X-ray crystallography, molecular modelling and molecular biology/biochemistry methods to determine the structure/function of these enzymes towards the goals of fundamental understanding of the metabolic pathways, and the generation of novel secondary metabolites with therapeutic potential

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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