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Award details
Dynamics and thermodynamics of recognition of ligands by the major urinary protein
Reference
B19388
Principal Investigator / Supervisor
Professor Steve Homans
Co-Investigators /
Co-Supervisors
Professor Simon Phillips
Institution
University of Leeds
Department
Inst of Molecular & Cellular Biology
Funding type
Research
Value (£)
209,544
Status
Completed
Type
Research Grant
Start date
01/10/2003
End date
30/09/2006
Duration
36 months
Abstract
This proposal concerns a study of the dynamics and thermodynamics of the interaction of the major urinary protein (MUP) with a panel of pyrazine-derived ligands, using isothermal titration calorimetry (ITC), x-ray crystallography, NMR and computational methods. The aim of this work is to characterise not only the enthalpic component of binding, but also the entropic component of binding for each ligand arising from dynamics of the ligand, protein and solvent. Preliminary ITC data on the panel of chosen ligands indicate that entropy is the dominant driving force for binding, and show intriguing enthalpy-entropy compensation phenomena that we hope to delineate by exploiting the sensitivity of high-resolution NMR to conformational entropy.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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