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The cytochrome c nitrite reductase: an integrated study of nitrite and nitric oxide reduction by a deca-heme enzyme complex

Reference	B18695
Principal Investigator / Supervisor	Professor David Richardson
Co-Investigators / Co-Supervisors	Professor Julea Butt, Dr Myles Cheesman, Professor Jeffrey Cole, Professor Andrew Hemmings
Institution	University of East Anglia
Department	Biological Sciences
Funding type	Research
Value (£)	354,500
Status	Completed
Type	Research Grant
Start date	20/10/2003
End date	19/07/2007
Duration	45 months

Abstract

Cytochrome c nitrite reductase (NrfA) catalyses the respiratory reduction of nitrite to ammonium. It is central to anaerobic energy conservation in many medically, agriculturally and industrially important bacteria. In Escherichia coli, NrfA scavenges substrates at the low nitrite concentrations that are physiologically relevant in many body fluids and also contributes to the detoxification of nitric oxide by reduction to ammonium. This research programme builds on structural and spectro-potentiometric characterisations of NrfA by integrating protein engineering, spectroscopy, voltammetry and X-ray crystallography to: (i) determine the mechanisms of nitrite and nitric oxide reduction at the active site and (ii) define the pathway(s) of the electron transfer to the active site.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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