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Mapping of membrane proteins by multi-frequency time domain EPR
Reference
B17596
Principal Investigator / Supervisor
Professor Andrew Thomson
Co-Investigators /
Co-Supervisors
Dr Myles Cheesman
Institution
University of East Anglia
Department
Chemistry
Funding type
Research
Value (£)
181,016
Status
Completed
Type
Research Grant
Start date
01/10/2002
End date
31/01/2006
Duration
40 months
Abstract
We plan to elucidate the mechanisms of the redox-linked proton pump of a respiratory quinol oxidase of E.coli, cytochrome bo3, carrying site directed nitroxide spin labels, by applying dual frequency, 9-95 GHz (X- and W-band), and time domain EPR to be installed at UEA. We seek mechanical motions or events triggered by the redox chemistry that drive the pump by studying the polarity and motional flexibility of proton uptake channels. At high field EPR measurement of g- anisotropy of a spin label allows separation of anisotropic label and protein motions. Pulsed EPR can measure 2D electron-electron double resonance (ELDOR) resolving different motional time scales and double quantum coherence for determination of distances between spins.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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