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An integrated approach to define the mechanism of respiratory nitrate reduction: spectroscopic and voltammetric studies
Reference
B17233
Principal Investigator / Supervisor
Professor Julea Butt
Co-Investigators /
Co-Supervisors
Professor David Richardson
Institution
University of East Anglia
Department
Chemistry
Funding type
Research
Value (£)
181,928
Status
Completed
Type
Research Grant
Start date
29/07/2002
End date
28/02/2006
Duration
43 months
Abstract
The molybdoprotein NarGHI is essential for the respiratory flexibility of many denitrifying, enteric and pathogenic bacteria. We have demonstrated that the nitrate reducing dimer, NarGH, catalyses nitrate reduction through two kinetically distinct pathways. These pathways are reversibly interconverted by a redox switch within the enzyme which may be provided by oxidation state changes of the molybdenum or [Fe-S] clusters within the enzyme. We now propose to distinguish between these possibilities and elucidate the mechanism of respiratory nitrate reduction at a molecular level through a combination of spectroscopic and voltammetric methodologies together with scanning probe microscopies.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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