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Early events in immunity protein folding investigated by microsecond mixing experiments

Reference	B17145
Principal Investigator / Supervisor	Professor Sheena Radford
Co-Investigators / Co-Supervisors	Professor David Smith
Institution	University of Leeds
Department	Inst of Molecular & Cellular Biology
Funding type	Research
Value (£)	193,788
Status	Completed
Type	Research Grant
Start date	01/01/2003
End date	31/12/2005
Duration	36 months

Abstract

Many proteins populate intermediates in the dead time of conventional stopped flow instruments. As a result, the nature of events that occur on submsec timescales cannot be analysed and their importance in folding remains unresolved. Here we propose a series of new experiments based on ultra-rapid mixing to provide the first insights into the early events of folding of two closely related proteins, Im7 and Im9. Specifically, we will determine (i) the conformational properties of early transition states and the mechanism formation of the on- pathway intermediate of Im7, (ii) the nature and importance of early events in folding of Im9 and (iii) the role of intermediates in shaping the energy landscape for folding of these proteins. The goal is to provide the first experimental measurements of the early search process in immunity protein folding so that theoretical models can be tested and developed.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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