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Structural characterisation of AAA molecules in transcriptional activation
Reference
B17129
Principal Investigator / Supervisor
Professor Xiaodong Zhang
Co-Investigators /
Co-Supervisors
Institution
Imperial College London
Department
Biological Sciences
Funding type
Research
Value (£)
121,740
Status
Completed
Type
Research Grant
Start date
13/01/2003
End date
12/01/2005
Duration
24 months
Abstract
Transcriptional activation is a fundamental process controlling gene expression. A large family of enhancer-dependent transcriptional activators including NtrC and PspF belongs to the AAA plus (ATPase Associated with various cellular Activities) super family of proteins. AAA plus proteins are required for numerous cellular activities in all life forms. The ATPase activity of the AAA plus transcription activators have been shown to target a key basal transcription complex and remodel it. Here we propose to characterise the nucleotide- dependent oligomerisation states of the AAA plus transcription activators, e.g. NtrC and PspF, as well as the associated holoenzyme using X-ray crystallography and cryo electron microscopy. This study would not only form foundations to understand the molecular mechanisms of the AAA plus transcriptional activators but also add essential structural information to the diverse AAA plus family.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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