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Exploring the high affinity kinetically selective binding of RNA over DNA by pyrrolidine-amide oligonucleotide mimics (POM)

Reference	B15998
Principal Investigator / Supervisor	Professor Jason Micklefield
Co-Investigators / Co-Supervisors
Institution	The University of Manchester
Department	Chemistry
Funding type	Research
Value (£)	207,632
Status	Completed
Type	Research Grant
Start date	07/01/2002
End date	07/01/2005
Duration	36 months

Abstract

Recently we have introduced a novel positively charged pyrrolidine-amide oligonucleotide mimic (POM) which binds with extremely high affinity to both DNA and RNA, whilst exhibiting a marked kinetic preference for RNA. We will develop an automated solid phase synthesis of mixed sequence POM and then investigate the thermodynamics and kinetics of POM binding to native nucleic acids, including duplex DNA and structured RNA. The research is of fundamental value in furthering our understanding of nucleic acid recognition. Furthermore oligonucleotide mimics like POM, with improved affinity and specificity for native nucleic acids, have significant potential both as therapeutic or diagnostic agents and as tools in molecular biology (e.g. microarrays). Hence the work is timely with the advent of the post genomics era.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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