BBSRC Portfolio Analyser
Award details
Directed evolution guided by catalytic promiscuity
Reference
B15990
Principal Investigator / Supervisor
Professor Florian Hollfelder
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Biochemistry
Funding type
Research
Value (£)
188,932
Status
Completed
Type
Research Grant
Start date
01/07/2002
End date
31/12/2005
Duration
42 months
Abstract
The objective of this research is to gain deeper insight into how enzymes bring about catalysis of two central bioreactions, phosphate and sulphate transfer. This project will use directed evolution approaches to find molecular solutions to travel the evolutionary distance between these two mechanistically related reactions. The choice of reactions is triggered by the observation of catalytic promiscuity, i.e. an enzyme activity in addition to the one it is evolved for and a possible starting point for natural evolution. In this way attempts will be made to mimic the way nature may have evolved new function. Kinetic and mechanistic experiments will enable the structural and quantitative mechanistic basis for the acquisition of a new catalytic activity to be traced.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search