Award details

Molybdopterin: synthesis; characterisation; metal binding; and catalytic properties in vitro and in vivo

Reference	B15270
Principal Investigator / Supervisor	Professor John Joule
Co-Investigators / Co-Supervisors	Dr David Collison
Institution	The University of Manchester
Department	Chemistry
Funding type	Research
Value (£)	174,740
Status	Completed
Type	Research Grant
Start date	01/04/2002
End date	30/09/2005
Duration	42 months

Abstract

Molybdopterin (MPT), the ligand of Mo or W at the catalytic centre of enzymes, will be synthesised and characterised. Dithiolene chemistry will be developed: analogues of the enzymes' catalytic centres will be produced for MPT and relatives; the physical and chemical properties of these systems will be determined. Binding of the synthetic MPT, including nuclear spin labels, to Cnx1 (a key protein in the biosynthesis of Moco in plants) and the incorporation of synthetic MPT into an enzyme will be studied. Binding of Mo and W by Cnx1 and insertion into MPT will be monitored. Studies with R. sphaeroides will produce W-substituted enzymes and determine how: i) MPT acquires a nucleotide and ii) two molecules of MPT are bound to the metal. (Joint with grant 42/B15275).

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

B15275 Molybdopterin: synthesis; characterisation; metal binding; and catalytic properties in vitro and in vivo

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