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Single molecule studies of protein folding using atomic force microscopy

Reference	B14774
Principal Investigator / Supervisor	Professor Sheena Radford
Co-Investigators / Co-Supervisors	Professor Tom McLeish, Professor David Smith
Institution	University of Leeds
Department	Inst of Molecular & Cellular Biology
Funding type	Research
Value (£)	246,936
Status	Completed
Type	Research Grant
Start date	01/08/2001
End date	01/08/2004
Duration	36 months

Abstract

Single molecule studies provide information that is not accessible to conventional ensemble measurements. In an ensemble experiment the heterogeneous sub-populations in structure, dynamics and function are obscured by averaging, but can be investigated by experiments which deal with molecules individually. Most importantly, only single molecule measurements allow a direct comparison with theory which often deals with biological systems at the single molecule level. We propose a series of experiments to analyse the structure and dynamics of different conformational states of proteins (native, denatured and partially folded) and the folding pathways between these states at the single molecule level using stomic force microscopy.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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