Award details

The crystallographic and kinetic delineation of the catalytic mechanism in fumarate reductase

Reference	B13402
Principal Investigator / Supervisor	Professor Malcolm Walkinshaw
Co-Investigators / Co-Supervisors	Professor Stephen Chapman, Professor Graeme Reid
Institution	University of Edinburgh
Department	Inst of Cell and Molecular Biology
Funding type	Research
Value (£)	174,088
Status	Completed
Type	Research Grant
Start date	01/10/2000
End date	01/10/2003
Duration	36 months

Abstract

We have solved the X-ray crystal structure of the S. frigidimarina fumarate reductase to 1.8 Angstrom resolution. This has allowed us to propose a detailed mechanism for fumarate reduction. We shall now build on this work by testing the proposed mechanism using a combination of site- directed mutagenesis, solution studies and X-ray crystallography. The crystallography will include inhibitor-bound structures and mutant enzyme structures. In addition, anaerobic crystallography will be used to examine the structure of the enzymes in the reduced as well as oxidised form. We believe this work will have important implications for all members of this important family of enzymes.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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