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The nature and importance of early events in protein folding investigated by microsec mixing experiments

Reference	B13374
Principal Investigator / Supervisor	Professor Sheena Radford
Co-Investigators / Co-Supervisors	Professor Colin Kleanthous, Professor David Smith
Institution	University of Leeds
Department	Inst of Molecular & Cellular Biology
Funding type	Research
Value (£)	173,086
Status	Completed
Type	Research Grant
Start date	06/12/2000
End date	06/12/2002
Duration	24 months

Abstract

Key to understanding how a protein folds is the need to determine the events occurring on a submsec timescale where, for many proteins, intermediates are generated and the native topology is sought and found. In this work we will build an apparatus capable of monitoring folding in microsec and use it to provide the first insights into the early stages in folding of a four helical protein, Im7, a small (86 residue) protein that folds in a three state mechanism. We will use the apparatus to determine 1) the role of the intermediate in folding; 2) the conformational properties of the early transition state; and 3) the mechanism of the early stages in folding by a series of mutagenesis and redesign experiments. The goal is to provide the first detailed views of the early search processes in helical protein folding such that theoretical models for folding can be tested and new theories and experiments developed.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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