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The structure and function of the internal ribosome entry site on the hepatitis c virus genome

Reference	B11731
Principal Investigator / Supervisor	Professor David Rowlands
Co-Investigators / Co-Supervisors	Dr Lucy Beales, Professor Andreas Kurt Hans Holzenburg, Dr Joachim Jaeger, Professor Susan Lea, Professor David Smith
Institution	University of Leeds
Department	Inst of Molecular & Cellular Biology
Funding type	Research
Value (£)	147,604
Status	Completed
Type	Research Grant
Start date	01/04/1999
End date	01/04/2002
Duration	36 months

Abstract

Initiation of hepatitis C virus translation involves direct association of ribosomes with a region in the 5' untranslated region of the genome known as the internal ribosome entry site (IRES). This mechanism of translation initiation is founded in some other viruses and very few cellular mRNAs and differs significantly from the cap dependent mechanism typical of the vast majority of cellular mRNAs. The IRESs have complex structures as predicted from sequence based algorithms supported by biochemical investigations and phylogenetic analyses. However, there is no direct information on the structure of these important RNA features. We intend to study the tertiary structure and biochemical/biophysical features of the HCV IRES using electron microscopy, atomic force microscopy and X-ray crystallography. Phage display technology will be employed to produce monoclonal antibodies specific to the HCV IRES to be used in each of the techniques described.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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