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Synthesis of phosphoinositides as biological probes for downstream protein effectors
Reference
B11586
Principal Investigator / Supervisor
Professor Andrew Holmes
Co-Investigators /
Co-Supervisors
Dr Phillip Hawkins
,
Professor Len Stephens
Institution
University of Cambridge
Department
Chemistry
Funding type
Research
Value (£)
170,356
Status
Completed
Type
Research Grant
Start date
01/10/1999
End date
31/12/2002
Duration
39 months
Abstract
Three key strands of an integrated programme involving Cambridge and Babraham will be followed. (i) natural and enantiomeric series of phospholipids, PtdIns3P, PtdIns(3,4,5)P3, PtdIns(3,5)P2 and PtIns (3,4)P2 will be synthesised and attached to beaded agarose solid supports for the screening and isolation of multidomain downstream binding proteins which will be characterised at Babraham; (ii) cell permeable phospholipids with photolabile protecting groups will be prepared and transported into cell membranes for release by laser flash photolysis/deprotection. An in depth study of intracellular release of phospholipids will be carried out; and (iii) the 5-phosphothionate analogue of PtdIns(3,4,5)P3 will be prepared and studied.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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