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Novel insights into folding mechanisms through studies of the family of bacterial immunity proteins

Reference	B11014
Principal Investigator / Supervisor	Professor Sheena Radford
Co-Investigators / Co-Supervisors	Professor Colin Kleanthous
Institution	University of Leeds
Department	Inst of Molecular & Cellular Biology
Funding type	Research
Value (£)	199,736
Status	Completed
Type	Research Grant
Start date	01/02/1999
End date	01/02/2002
Duration	36 months

Abstract

We will elucidate the relationship between amino acid sequence, structure and folding mechanism using the family of bacterial immunity proteins. These proteins have identical folds and highly homologous sequences. Nevertheless, our preliminary data shows that the proteins fold by distinct mechanisms. We will (i) deduce the folding mechanisms of the four E-type immunity proteins; (ii) investigate the conformation of intermediates and transition states and (iii) use random mutagenesis to generate new sequences commensurate with this fold and biophysical methods to determine their folding properties. The goal is to probe the energy landscape for folding this four helix bundle and identify residues that differentiate their folding mechanisms.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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