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Nitrous oxide reductase: comparative and functional studies of novel copper-thiolate centres
Reference
B08413
Principal Investigator / Supervisor
Professor Andrew Thomson
Co-Investigators /
Co-Supervisors
Professor Benjamin Berks
Institution
University of East Anglia
Department
Chemistry
Funding type
Research
Value (£)
162,995
Status
Completed
Type
Research Grant
Start date
06/07/1998
End date
31/05/2002
Duration
46 months
Abstract
To undertake a spectroscopic and kinetic study of nitrous oxide reductase from three different organisms (Pseudomonas stutzeri, Paracoccus denitrificans and Alcaligenes xylosoxidans) in order to extend and verify the current working model of the nature of the metal centres. This programme will (i) control the form of the enzyme as a function of both growth conditions and purification procedures, (ii) obtain direct spectroscopic evidence for the nature of the active site by using low temperature magnetic circular dichroism and electron paramagnetic resonance spectroscopies and by extending the characterisation to include resonance Raman, low temperature luminescence and mass spectroscopy, (iii) carry out spectroscopic characterisation of the interaction of the action site of both wild type and mutants forms of the enzyme with exogenous ligands and inhibitors.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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