Award details

The structural and mechanistic mapping of a unique fumarate reductase

Reference	B07650
Principal Investigator / Supervisor	Professor Stephen Chapman
Co-Investigators / Co-Supervisors	Professor Graeme Reid, Professor Malcolm Walkinshaw
Institution	University of Edinburgh
Department	Sch of Chemistry
Funding type	Research
Value (£)	178,365
Status	Completed
Type	Research Grant
Start date	01/04/1997
End date	01/04/2000
Duration	36 months

Abstract

The fumarate reductase from Shewanella putrefaciens is a unique flavo-haemo-enzyme. In contrast to the typical membrane bound fumarate reductases from other microorganisms, the S. putrefaciens enzyme is structurally simpler, soluble, and available in large amounts. This makes it immensely valuable for structural and functional studies. We shall therefore: i) Determine the X-ray crystal structure of the enzyme; ii) Provide recombinant wild-type and mutant forms of the enzyme in sufficient quantities for full structure/function analysis; and iii) Determine the exact catalytic mechanism of the enzyme at a molecular level .

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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