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Design of proteins of prescribed structure: core-directed protein design
Reference
B04756
Principal Investigator / Supervisor
Professor Dek Woolfson
Co-Investigators /
Co-Supervisors
Dr Jonathan Cox
Institution
University of Sussex
Department
Biology and Environmental Science
Funding type
Research
Value (£)
143,746
Status
Completed
Type
Research Grant
Start date
01/10/1995
End date
01/10/1998
Duration
36 months
Abstract
Attempts to design proteins de novo tend to produce molecules that contain secondary structure but lack fixed tertiary interactions. We describe a new approach to combat this failing. The method focuses on obtaining the set of hydrophobic amino acids that best specifies, or packs, the core of a given protein topology. The proposed procedure will involve: (1) Random mutagenesis at the hydrophobic core positions of the target protein. (2) Display of the resulting pool on the surface of phage. (3) Proteolytic selection to remove unfolded molecules from folded ones. (4) Affinity chromatography to separate intact protein-phage fusions from the other debris. (5) DNA sequencing to determine the primary structures of competently folded proteins.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Biological Chemistry Initiative (BCI) [1995]
Funding Scheme
X – not Funded via a specific Funding Scheme
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