BBSRC Portfolio Analyser
Award details
The interaction of DNA gyrase with FdhE a protein involved in the assembly of the formate dehydrogenase complex
Reference
9810615
Principal Investigator / Supervisor
Professor Anthony Maxwell
Co-Investigators /
Co-Supervisors
Institution
John Innes Centre
Department
Biological Chemistry
Funding type
Research
Value (£)
138,336
Status
Completed
Type
Research Grant
Start date
01/03/1999
End date
14/07/2002
Duration
40 months
Abstract
DNA gyrase is a type II topoisomerase from bacteria which controls the level of DNA supercoiling. Using affinity columns we have found evidence for specific interaction between the gyrase A subunit and FdhE, a protein involved in the assembly of the formate dehydrogenase complex in E. coli. Experiments in vitro show that TdhE can inhibit gyrase-catalysed DNA supercoiling and DNA binding. We have now made a fusion construct which expresses FdhE and plan to investigate its potential role in the regulation and control of the physiology and enzymology of DNA gyrase.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
ROPA 1998 (ROPA1998) [1998]
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search