BBSRC Portfolio Analyser
Award details
Engineering novel fatty acid desaturases
Reference
WCP11835
Principal Investigator / Supervisor
Professor Johnathan Napier
Co-Investigators /
Co-Supervisors
Institution
Rothamsted Research
Department
Plant Pathology and Microbiology
Funding type
Research
Value (£)
142,002
Status
Completed
Type
Research Grant
Start date
10/01/2000
End date
10/01/2003
Duration
36 months
Abstract
We wish to demonstrate the evolutionary relationship between the plant sphingolipid desaturases and the front-end fatty acid desaturases, the latter being important for the synthesis of nutraceutical compounds such as GLA and arachidonic acid. This will be achieved by mutageneisis of the sphingolipid desaturase, to convert it to a D6 fatty acid desaturase. Functional analysis will be carried out by expression in yeast and Arabidopsis, followed by GC and GCMS of lipid fractions. We will also generate novel fatty acid desaturases by using DNA shuffling of cytoplasmic domains of the sphingolipid desaturase. Identification of new fatty acid desaturase activities generated by shuffling will be selected by a yeast complementation assay.
Summary
unavailable
Committee
Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Wealth Creating Products of Plants Initiative (WCP) [1995-1999]
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search