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Use of potato virus X for high level production of foreign proteins in plants

Reference	WCP05440
Principal Investigator / Supervisor	Dr Simon Santa Cruz
Co-Investigators / Co-Supervisors	Dr Sean Chapman, Professor Karl Oparka
Institution	The James Hutton Institute
Department	Division of Pathology
Funding type	Research
Value (£)	225,021
Status	Completed
Type	Research Grant
Start date	01/01/1996
End date	01/09/1999
Duration	44 months

Abstract

We have shown the production of stable virions that are decorated along their surface with a passenger protein, by fusion of a 27 kDa protein to the N-terminus of the potato virus X (PVX) coat protein (CP). The modified virus moved systematically and accumulated to high levels. This indicates that PVX could be used as a vector for production of foreign proteins in plants. Further modified forms of PVX, encoding translational fusions between the CP and foreign proteins, will be produced to define the parameters of the system. These mutants will be tested for the ability to assemble and to accumulate in plants. Strategies will be developed to maximise the size of passenger proteins and to facilitate isolation of the foreign proteins from virus.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Wealth Creating Products of Plants Initiative (WCP) [1995-1999]
Funding Scheme	X – not Funded via a specific Funding Scheme

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