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Development of environmental scanning electron microscopy for structural analysis of cellular systems

ReferenceSYS04681
Principal Investigator / Supervisor Professor Athene Donald DBE FRS
Co-Investigators /
Co-Supervisors
Institution University of Cambridge
DepartmentPhysics
Funding typeResearch
Value (£) 141,070
StatusCompleted
TypeResearch Grant
Start date 01/11/1995
End date 01/11/1998
Duration36 months

Abstract

Environmental scanning electron microscopy is a comparatively new technique which permits the imaging of samples whilst maintained in a fully hydrated state. We will study the deformation of cell walls in situ, whilst controlling the state of hydration to simulate drying. In addition, it will be possible to partially cook materials such as potatoes in situ, and then retest the sample to explore the changing mechanical properties. Finally we will study starch granule structure, concentrating on the possibility of imaging samples pre-stained with iodine without the iodine evaporating as occurs in conventional SEM's in order to locate the amylose.

Summary

unavailable
Committee Closed Committee - Engineering & Biological Systems (EBS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative Systems - ROPA (SYS) [1994]
Funding SchemeX – not Funded via a specific Funding Scheme
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