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Determination of the three-dimensional structures of membrane transport proteins and of their ligand-protein complexes
Reference
SBD07613
Principal Investigator / Supervisor
Professor Peter Henderson
Co-Investigators /
Co-Supervisors
Dr R Herbert
,
Dr Mark Parsons
Institution
University of Leeds
Department
Inst of Molecular & Cellular Biology
Funding type
Research
Value (£)
207,104
Status
Completed
Type
Research Grant
Start date
01/11/1997
End date
01/11/2000
Duration
36 months
Abstract
The primary aim is to elucidate the 3D structures and molecular mechanisms of the integral membrane proteins for transport of xylose-H+ found in Escherichia coli and for `multi-drug' efflux found in Bacillus subtilis. Both are paradigms for numerous transporters found in diverse organisms from microbe to man. The principal techniques are: 3D crystallisation trials on each protein, followed by X-ray diffraction; novel solid state NMR to elucidate the kinetics of ligand-protein interaction and the structures of the ligand binding sites; and 2D crystallisation in membranes followed by electron diffraction. Mass spectrometry and numerous biochemical, genetical, immunochemical, and biophysical methods may be used also.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Structural biology and design applications (SBD) [1996]
Funding Scheme
X – not Funded via a specific Funding Scheme
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