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High-resolution structures of extracellular respiratory proteins by electron cryomicroscopy and angular reconstitution

ReferenceSBD07611
Principal Investigator / Supervisor Professor Marin Van Heel
Co-Investigators /
Co-Supervisors
Institution Imperial College London
DepartmentBiological Sciences
Funding typeResearch
Value (£) 175,560
StatusCompleted
TypeResearch Grant
Start date 05/06/1997
End date 05/06/2000
Duration36 months

Abstract

We seek to determine the structures of large extracellular respiratory proteins, including the keyhole limpet (Megathura crenulata) haemocyanin (KLH: a strong immunostimulant) and the haemoglobin of Lumbricus terrestris, to resolutions better than - 10 angstron using cryomicroscopy and angular reconstitution. The reconstructions will be interpreted by fitting known X-ray structures of domains and/or subunits of related proteins. In addition to elucidating the structures and possibly the structure-function relations of these intriguing, allosteric proteins, we also want to establish the single-particle methodology as a fast and reproducible way to visualise alpha -helics within large oligomeric proteins.

Summary

unavailable
Committee Closed Committee - Biomolecular Sciences (BMS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative Structural biology and design applications (SBD) [1996]
Funding SchemeX – not Funded via a specific Funding Scheme
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