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2D and 3D approaches to the crystal structure of bacterial membrane proteins - cytochrome bd oxidases and a tetracycline transporter

Reference	SBD07582
Principal Investigator / Supervisor	Professor Per Bullough
Co-Investigators / Co-Supervisors	Professor Robert K Poole, Professor Adrian Walmsley
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	142,116
Status	Completed
Type	Research Grant
Start date	01/05/1997
End date	01/01/2001
Duration	44 months

Abstract

Support is requested to `pump-prime' a major new initiative in membrane protein structure at the Krebs Institute, Sheffield. We will explore methods for the 2D and 3D crystallisation of bacterial membrane proteins, namely; a tetracycline transporter and two different cytochrome bd-type oxidases. The emphasis will be on 2D crystallisation. Although the rate of progress in this area over a 3 year period cannot be predicted we aim to lay the ground work for a successful crystallisation and structure determination of at least one of the proteins. This would then form the basis of a further project proposal for structure determination, by cryoelectron microscopy or X-ray crystallography. This work will further our general understanding of membrane protein structure and the specific structure/function relationships of some scientifically, medically and economically important proteins.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Structural biology and design applications (SBD) [1996]
Funding Scheme	X – not Funded via a specific Funding Scheme

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