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Engineering the heavy chain constant region of human IgG to modulate glycosylation and effector functions
Reference
SBD07526
Principal Investigator / Supervisor
Professor R Jefferis
Co-Investigators /
Co-Supervisors
Institution
University of Birmingham
Department
Medical Sciences - Medicine
Funding type
Research
Value (£)
233,940
Status
Completed
Type
Research Grant
Start date
01/08/1997
End date
01/08/2000
Duration
36 months
Abstract
Oligosaccharide homogeneity and consistency poses significant problems for the production of recombinant glycoproteins intended for therapeutic use. We aim to use protein engineering to design antibody constructs that will direct glycosylation towards homogeneity with or without compromised biological activities such as phagocytosis, complement-mediated lysis, or biological half-life. Oligosaccharide structures will be analysed by HPLC and MALDI-TOF mass spectrometry. We will define the minimal structural unit of Fc that will mediate selected effector functions, expressing the CH2 domain in different protein environments, for eventual expression in fusion proteins.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Structural biology and design applications (SBD) [1996]
Funding Scheme
X – not Funded via a specific Funding Scheme
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