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Towards targeted protein interactions: defining a TPR-mediated interaction and exploiting sequence degeneracy for novel biomolecular design
Reference
SBD07486
Principal Investigator / Supervisor
Dr Johanna Avis
Co-Investigators /
Co-Supervisors
Institution
The University of Manchester
Department
Life Sciences
Funding type
Research
Value (£)
41,382
Status
Completed
Type
Research Grant
Start date
01/06/1997
End date
01/06/1998
Duration
12 months
Abstract
The TPR motif is an evolutionary conserved motif involved in diverse cellular processes, thought to mediate protein-protein interactions. A model system for studying TPR-mediated interactions is provided by the yeast CDC23 and CDC27 proteins, components of the anaphase promoting complex (APC). The solution structure of a TPR motif of CDC27, essential for interaction with CDC23 will be solved. Deletion mapping will define the minimal region of CDC23 required for the interaction. Further NMR experiments will provide the first detailed insight into a TPR-mediated interaction. We shall then exploit the structural information and the sequence degeneracy displayed by TPR motifs to explore the feasibility of using a forced molecular evolution approach to design a new TPR motif with new, defined, substrate binding properties.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Structural biology and design applications (SBD) [1996]
Funding Scheme
X – not Funded via a specific Funding Scheme
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