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Using yeast molecular genetics to determine the importance of base-excision repair to the survival of oxidative stress and ageing

Reference	SAG09922
Principal Investigator / Supervisor	Professor Peter William Piper
Co-Investigators / Co-Supervisors
Institution	University College London
Department	Structural Molecular Biology
Funding type	Research
Value (£)	139,808
Status	Completed
Type	Research Grant
Start date	01/11/1998
End date	01/11/2001
Duration	36 months

Abstract

Mammalian studies have given strong indications that damage due to reactive oxygen species (ROS) is important in the progressive function losses associated with ageing. The extents to which DNA repair counteracts ROS damage and thus senescence has never been studied, even though oxidative DNA damage is widely implicated in ageing. This study will use yeast mutants either lacking or overexpressing specific DNA glycosylases and mitochondrial DNA repair activities to investigate if oxidative DNA damage influences lifespan and sensitivity to oxidants. By deletion or overexpression of either the cytoplasmic or the mitochondrial superoxide dismutase in these same cells, the relative contributions of nuclear and mitochondrial DNA damage will also be assessed. The aim will be to obtain conclusive evidence as to whether or not there is a casual relationship between ROS production, DNA damage and ageing.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Initiative on Science of Ageing (SAG) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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