Award details

A strategy for cloning genes responsible for cellular senescence

ReferenceSAG09921
Principal Investigator / Supervisor Dr Ian Kill
Co-Investigators /
Co-Supervisors
Institution Brunel University London
DepartmentBiological Sciences
Funding typeResearch
Value (£) 100,762
StatusCompleted
TypeResearch Grant
Start date 01/10/1998
End date 01/04/2000
Duration18 months

Abstract

The aim of the work is to use a cell-free extract of S-phase human cells as a bioassay to detect activities derived from senescent cells which prevent DNA replication. Senescent cell-derived inhibitory activities will be obtained initially from cytosolic extracts of senescent cells and subsequently from a pool of proteins expressed en masse from a cDNA library of senescent cell- specific mRNAs. Senescent cell extracts will be tested for ability to inhibit specific cylin-dependent kinase activities in vitro. Completion of the project will provide a reliable bioassay for selective screening of cDNA libraries to clone genes involved in cellular ageing.

Summary

unavailable
Committee Closed Committee - Genes & Developmental Biology (GDB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative Initiative on Science of Ageing (SAG) [1998]
Funding SchemeX – not Funded via a specific Funding Scheme
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