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Trout interferon regulatory factors: bioactivity and involvement in interferon signalling

Reference	S20314
Principal Investigator / Supervisor	Professor Chris Secombes
Co-Investigators / Co-Supervisors	Dr Bertrand Collet
Institution	University of Aberdeen
Department	Inst of Biological and Environmental Sci
Funding type	Research
Value (£)	180,013
Status	Completed
Type	Research Grant
Start date	01/04/2004
End date	31/03/2007
Duration	36 months

Abstract

Interferon Regulatory Factors (IRF) are nuclear DNA-binding molecules which play a major role in the regulation of interferon (IFN) activity. In humans, they act by activating/repressing the expression of IFN and IFN-induced genes. We have cloned two IRF genes in rainbow trout and in this study will examine their function: i) by producing the recombinant proteins and analysing their DNA binding ability and specificity by ElectroMobility Shift Assay (EMSA); ii) by determining the involvement of IRF in IFN signalling by RNA interference and over-expression in cell lines and following DNA injection into muscle in vivo. The subsequent alteration of IFN activity will be assessed by expression analysis of IFN-induced genes such as Mx.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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