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The role of Rho family GTPases in osteoblast differentiation and bone formation
Reference
S19521
Principal Investigator / Supervisor
Professor Agamemnon Grigoriadis
Co-Investigators /
Co-Supervisors
Institution
King's College London
Department
Craniofacial Dev Orthodon and Microbiol
Funding type
Research
Value (£)
216,336
Status
Completed
Type
Research Grant
Start date
01/03/2004
End date
31/05/2007
Duration
39 months
Abstract
The signalling pathways that enable bone-forming osteoblasts to respond to extracellular signals and form bone are poorly understood. We have previously observed that activation of the small GTPase Rho inhibits, whereas inhibition of Rho kinase (ROK) stimulates bone formation in vitro. Here, we will demonstrate that altered Rho activity regulates bone formation in vitro and in vivo. We will identify the effects of activators and inhibitors of Rho signalling on osteoblast gene expression in vitro and on bone formation in ex vivo calvarial explants. Finally, we will generate transgenic mice with dominant active or dominant negative Rho proteins targeted to osteoblasts, to demonstrate the importance of Rho-ROK signalling in bone formation.
Summary
unavailable
Committee
Closed Committee - Animal Sciences (AS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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