Award details

The role of sortase-anchored proteins in the virulence of Streptococcus uberis

Reference	S19514
Principal Investigator / Supervisor	Professor James Leigh
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	Div of Immunology Compton
Funding type	Research
Value (£)	175,676
Status	Completed
Type	Research Grant
Start date	01/04/2004
End date	31/03/2006
Duration	24 months

Abstract

Sortase (Srt) is a transamidase required to anchor proteins covalently at the surface of Gram-positive bacteria. Mutagenesis will be used to inactivate Srt activity in S. uberis. Proteomics will be used to identify proteins anchored at the bacterial surface through Srt. Interrogation of the genomic sequence will enable the orfs of Srt-anchored proteins to be identified and compared to a list of 30 putative sortase substrates, identified directly from the genome. The ability of srt mutant to display properties associated with virulence and to induce mastitis in the target species will be determined to identify the collective role of Srt-anchored proteins in the virulence of S. uberis and the pathogenesis of bovine mastitis.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

S19514/2 The role of sortase-anchored proteins in the virulence of Streptococcus uberis

S19514/3 The role of sortase-anchored proteins in the virulence of Streptococcus uberis

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