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Regulation of zinc by the zebrafish gill: a functional genomics approach

Reference	S19512
Principal Investigator / Supervisor	Professor Christer Hogstrand
Co-Investigators / Co-Supervisors	Professor Peter Kille
Institution	King's College London
Department	Nutritional Sciences c
Funding type	Research
Value (£)	418,356
Status	Completed
Type	Research Grant
Start date	02/10/2003
End date	01/12/2006
Duration	38 months

Abstract

To fish, zinc (Zn) is both a micronutrient and a toxicant of high importance. The gill epithelium regulates uptake of Zn in response to changes in Zn availability. We will use microarray to identify pathways that coordinate Zn homeostasis in the zebrafish gill. Expression of 14K genes, including all known Zn regulatory zebrafish genes, will be monitored in the gill during acclimation to Zn regimes ranging from deficient to excess. Temporal patterns of gene expression will be linked to Zn status, branchial Zn transport and metabolic effects. Search for the regulatory elements involved will be performed by promoter comparison of clustered genes. This will be followed by localisation of key transcripts among gill cell types. In parallel experiments, expression of genes implicated in the Zn regulatory pathways will be manipulated pharmacologically and by RNAi silencing.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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