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Identification and regulation of distinct genetic pathways involving separate glucocorticoid receptors in fish
Reference
S18960
Principal Investigator / Supervisor
Professor Nicolas Bury
Co-Investigators /
Co-Supervisors
Institution
King's College London
Department
Pharmacy
Funding type
Research
Value (£)
177,960
Status
Completed
Type
Research Grant
Start date
02/06/2003
End date
01/06/2006
Duration
36 months
Abstract
The physiological significance of teleost fish possessing two glucorticoid receptors (GR1 and GR2) that are widely expressed but possess different transactivational sensitivities to cortisol has not been ascertained. The present study uses gill and hepatocyte primary cell culture to determine different genetic pathways regulated by the two GRs in rainbow trout. The mechanisms for the control of specific receptor gene expression will be assessed by: 1) identification of the upstream regulatory regions of GR1 or GR2 target genes by chromatin immunoprecipitation; 2) determining the functional significance of amino acid sequence differences between both GRs in regions involved in transactivation and DNA binding, and 3) assessing the influence of receptor homo- or hetero-dimer formation on transactivation efficiency.
Summary
unavailable
Committee
Closed Committee - Animal Sciences (AS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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