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Technology development: a mouse model of the hamster tau mutation to study integrative circadian physiology

ReferenceS18856
Principal Investigator / Supervisor Professor Andrew Loudon
Co-Investigators /
Co-Supervisors
Professor Raymond Boot-Handford, Professor Hugh Piggins
Institution The University of Manchester
DepartmentLife Sciences
Funding typeResearch
Value (£) 314,116
StatusCompleted
TypeResearch Grant
Start date 17/11/2003
End date 16/11/2006
Duration36 months

Abstract

Circadian clocks require biochemical regulation of time-delays in transcriptional feedback loops, which confer 24h rhythmicity. Genetic studies reveal casein kinase-1 epsilon (CK1e) as a core component, and a mutation in hamsters of CK1 epsilon impacts on physiology of central and peripheral oscillators. We aim to dissect circadian behaviour by developing both a CK1 epsilon tau mutant and a CK1 epsilon null mutant mouse, and define the impact of these mutations on circadian oscillators regulating behaviour and cyclical gene expression of canonical clock genes. The targeting strategy involves both a null mutation substitutional approach, obviating potential difficulties associated with potential lethality of null CK1 epsilon animals.

Summary

unavailable
Committee Closed Committee - Animal Sciences (AS)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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